Connection guide

PepSep

As for all columns used in LCMS applications, you get the best performance from the PepSep columns if connections between the LC system, the column and the emitter are made correctly and with the correct fittings.


Below you will find suggestions to connectors giving proper connections between the column, the LC and the emitter if correctly installed. Please follow the suppliers installation guides when making the connections.


The recommended connectors and sleeves all come from IDEX and can be found here www.idex-hs.com But there are similar products offered from other vendors which give the same high performance.

Connection between the LC and the column.

When connecting the column to the LC system, it is important to choose a connector that is compatible with the pressure applied by the LC system to the column. If improper connections are made, leaks and void volumes might cause poor chromatography.

If your connecting capillary is a fused silica OD 360 µm and you want to connect to a PepSep column without the UHP fitting, the following unions can be used:

Connector

Pressure rating psi/bar

PN

Comment

MicroTight Union

5000/345

P-772



VHP MicroTight Union

15000/1034

UH-436



MicroTight Union 1/32

5000/345

P-771

Used with F-385 PEEK sleeves on capillary and column

VHP MicroTight Union 1/32*

15000/1034

UH-632

Used with F-385 PEEK sleeves on capillay and column

*Use this connector if higher pressures than 5000 psi/345 bar is needed. The connector is not rated to 15000 psi/1034 bar when used with a PEEK sleeve

If your connecting capillary is a fused silica OD 360 µm and you want to connect the column is using a venting tee as e.g with the EASY-nLC, the following connectors can be used.

Connector

Pressure rating psi/bar

PN

Comment

MicroTee PEEK

5000/345

P-888



VHP MicroTee

15000/1034

UH-750



MicroTee PEEK 1/32


5000/345

P-885

Used with F-385 PEEK sleeves an capilaries and column

VHP MicroTee 1/32*


15000/1034

UH-700

Used with F-385 PEEK sleeves on capillaries and column

*Use this connector if higher pressures than 5000 psi/345 bar is needed. The connector is not rated to 15000 psi/1034 bar when used with a PEEK sleeve

If your connecting capillary is a fused silica OD 360 µm and you want to connect with a cross, for venting and connection of a Liquid Junction as e.g with the EASY-nLC, the following connectors can be used.

Connector

Pressure rating psi/bar

PN

Comment

MicroCross PEEK

5000/345

P-889




VHP MicroCross

15000/1034

UH-750




MicroCross PEEK 1/32

5000/345

P-887

Used with F-385 PEEK sleeves on capillaries and column


VHP MicroCross 1/32*

15000/1034

UH-702

Used with F-385 PEEK sleeves on capillaries and column


*Use this connector if higher pressures than 5000 psi/345 bar is needed. The connector is not rated to 15000 psi/1034 bar when used with a PEEK sleeve

If your connecting capillary is a PEEK-Sil OD 1/32 inch and you want to connect to a PepSep column without the UHP fitting, the following unions can be used.

Connector

Pressure rating psi/bar

PN

Comment

MicroTight Union 1/32

5000/345

P-771

Used with F-385 PEEK sleeve on the column

VHP MicroTight Union 1/32*

15000/1034

UH-632

Used with F-385 PEEK sleeve on the column

*Use this connector if higher pressures than 5000 psi/345 bar is needed. The connector is not rated to 15000 psi/1034 bar when used with a PEEK sleeve

If your connecting capillary is a PEEK-Sil OD 1/32 inch and you want to connect a PepSep column with a venting tee, as e.g with the EASY-nLC, the following connectors can be used.

Connector

Pressure rating psi/bar

PN

Comment

MicroTee PEEK 1/32



5000/345

P-885

Used with F-385 PEEK sleeve on the column

VHP MicroTee 1/32*



15000/1034

UH-700

Used with F-385 PEEK sleeve on the column

*Use this connector if higher pressures than 5000 psi/345 bar is needed. The connector is not rated to 15000 psi/1034 bar when used with a PEEK sleeve

If your connecting capillary is a PEEK-Sil OD 1/32 inch and you want to connect a PepSep column with a cross for venting and connection of a liquid junction, as e.g with the EASY-nLC, the following connectors can be used.

Connector

Pressure rating psi/bar

PN

Comment

MicroCross PEEK 1/32



5000/345

P-885

Used with F-385 PEEK sleeve on the column

VHP MicroCross 1/32*



15000/1034

UH-700

Used with F-385 PEEK sleeve on the column

If your connecting capillary has a nut with a 10-32 thread, like e.g. nanoViper tubing, you can connect directly to the PepSep columns with a pre-mounted UHP feemale connector.

If you prefer to connect a PepSep column without the UHP union to a 10-32 thread nut, e.g. nanoViper, you can use the following unions.

Connector

Pressure rating psi/bar

PN

Comment

MicroTight Adaptor 1/16 - 360

5000/345

P-882


Microtight Adaptor 1/16 - 1/32

5000/345

P-881

Used with F-385 PEEK sleeve on the column

VHP MicroTight Adaptor 1/16 - 360

12000/827

UH-634


Adaptor UHP 1/16 - 1/32*

15000/1034

UH-881

Used with F-385 PEEK sleeve on the column

*Use this connector if higher pressures than 5000 psi/345 bar is needed. The connector is not rated to 15000 psi/1034 bar when used with a PEEK sleeve

If the column is going to be used with a venting tee as e.g with the EASY-nLC, the following connectors can be used.

Connector

Pressure rating psi/bar

PN

Comment

VHP Micro Adaptor Tee 1/16 - 360

15000/1035

UH-753


Connection between column and emitter:

The column and emitter connects diferently in different ion sources.

To avoid peak broadening and peak tailing, dead volumes (voids) between the column and the emitter must be minimized.

Therefore we recommend to use a true zero dead volume (ZDV) connector which makes a butt connection between the column and the emitter.

In addition to a proper connector it is also important to choose the correct sleeve when making the connection.

Below are examples of connectors and sleeves connecting the PepSep column and emitter so best possible performance is achieved.

Connection between a PepSep column and an emitter with OD 150 µm

Connector

PN

Sleeve (column)/(emitter)

PN/PN

MicroTight Union 1/32

P-771

(1/32-360)/(1/32-150)

F-385/F-381


VHP MicroTight Union 1/32

UH-632

(1/32-360)/(1/32-150)

F-385/F-381

PepSep

Sarumgaardsvej 4

5290 Marslev

Denmark

Phone: +4565952009

E-mail address: contact@pepsep.com


VAT no. DK35555773