The Columns

All columns are packed in Polyimide coated Fused Silica with an outer diameter (OD) at 360 µm.

To ensure smooth blunt ended connections between transfer lines, column and spray emitters, the column entrance and exit are finely polished and individually inspected in microscope.

To minimize post-column eluent mixing, the precise positioned potassium silicate frit is as short as possible, 250-350 µm.

Potassium silicate frit positioned in a 360 µm fused silica

Separation Columns

The separation columns are packed with ReproSil 1,5 µm, 1,9 µm or 3 µm C18 beads, pore diameter 120Å.

They come in various lengths (L) and inner diameters (ID) and with or without connecting unions.

Use the Column configurator to get part numbers and prices for our standard columns.

If you have a special need, which our range of standard columns does not cover, columns can be custom made. Mail with information’s of the dimensions and connections you need and we will send you an offer.

Columns with pre-mounted UHP and nanoConnect connections.

The separation columns can be bought with pre-mounted unions.

For safe and easy connection to the high pressure end of the column we have a 10-32 thread female union making connedtion to most LC systems, with 1/16 connectors, easy and precise. The UHP connection is tested to be used 1000 bar.

In the low pressure end we have a nanoConnect connector with a 10-32 thread male union making connection to any 10-32 thread receiving union easy and reliable. The nanoConnect system ensures a zero dead volume connection to any but connected emitter connected to a 10-32 thread union.

Separation column with UHP union and nanoConnect

Separation column with nanoConnect

Separation column with UHP union

Trap Columns

The trap columns are packed with ReproSil 3 µm or 5 µm C18 beads, pore diameter 120Å.

They come with a column length (CL) of 2 cm. Total length (L) of the capillary is 6 cm.

The inner diameters (ID) are 100 or 150 µm


Emitters with integrated liquid junction

Emitters with 1/32" PEEK sleeves

The emitters comes in two versions. One is with integrated liquid junction which is to be used with the PepSep sprayer. The other version, with 1/32" PEEK sleeve, is to be used in custom made setups and comes in 3 lengths 3 ,4 and 5 cm.

All emitters have an OD at 150 µm and comes as either stainless steel or fused silica.

The steel emitter has an ID at 30 µm.

The fused silica emitters have two dimensions, either ID 10 µm or ID 20 µm.

Both types of emitters are polished with fine diamond films to give a perfect tapered tip.

Stainless steel emitter with liquid junction

Fused silica emitter with liquid junction

Stainless steel emitter

Fused silica emitter

PepSep Sprayers

PepSep Sprayer 1

PepSep Sprayer 2

The PepSep Sprayers connects the new emitter, with the integrated liquid junction, and the PepSep columns, with the nanoConnect outlet, so a perfect match is made. The perfect match ensures a zero dead volume connection giving the best possible chromatographic performance.

More info about the PepSep sprayers, the emitter with the integrated liquid junction and the installation of emitter and column can be found on the PepSep Sprayers page

HV cable connecting the Thermo sources to a banana plug

Croc with banana plug